Abstract:

BACKGROUND: Sustained release of bone morphogenetic proteins (BMPs) at stable speed is a prerequisite for osteogenic induction. Therefore, a suitable carrier for the sustained release of BMPs is required. However, there is no ideal carrier for BMPs as yet.
OBJECTIVE: To explore the sustained release process of BMP-7 and osteogenic activity in the xenogeneic bone composite that is the compound of xenogeneic bone, recombinant human BMP-7 (rhBMP-7) and fibrin sealant (FS).
METHODS: With the xenogeneic cattle spongy bone as a scaffold material, the solution containing rhBMP-7 was adsorbed onto the scaffold by means of vacuum negative pressure absorption, and fibrin sealant (FS) was sprayed onto the surface of the scaffold. The xenogeneic bone composite was made up of these three materials as sustained release materials. All scaffold materials were randomly divided into three groups: blank group (the scaffold material was xenogeneic bone with no rhBMP-7 and FS), control group (the scaffold material was xenogeneic bone with rhBMP-7) and experimental group (the scaffold material was the composite material of xenogeneic bone, rhBMP-7 and FS). All samples were immerged into phosphate buffer solution for 28 continuous days, and the release levels of rhBMP-7 from the samples were measured by high performance liquid chromatography at various set time. All the scaffold materials were co-cultured with passage 3 bone marrow mesenchymal stem cells. Alkaline phosphatase activity, osteopontin and osteocalcin expression levels were detected at days1, 3, 7, and 14 after co-culture
RESULTS AND CONCLUSION: rhBMP-7 was not detected in the blank control group, and the sustained release of rhBMP-7 in the experimental group was better than that in the control group. The alkaline phosphatase activity, osteopontin and osteocalcin levels were ranked as follows: blank control group < control group < experimental group, and there was a significant difference between groups (P < 0.05). These findings indicate that the xenogeneic bone/rhBMP-7/FS composite material as a good sustained-release carrier of BMP-7 shows strong osteogenic activity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Protein 7, Fibrin Tissue Adhesive, Delayed-Action Preparations, Tissue Engineering